The PYHIN1 Polyclonal Antibody (PACO36818) is a vital tool for researchers studying PYHIN1, a protein involved in immune response and inflammation. This antibody, produced through rabbit immunization, demonstrates high specificity and sensitivity when tested with human samples, making it a reliable option for Western blot experiments. By binding to the PYHIN1 protein, this antibody enables researchers to detect and analyze PYHIN1 expression in various cell types, facilitating research in immunology and inflammatory diseases.
PYHIN1, also known as a member of the interferon-inducible PYHIN protein family, is involved in the innate immune response by sensing pathogen-derived DNA and activating inflammatory pathways. Its role in immune signaling makes it a promising target for studying conditions like autoimmune disorders, infectious diseases, and cancer. Investigating the function of PYHIN1 is crucial for developing therapeutic strategies that modulate the immune response in these pathological contexts.
Antibody Name:
PYHIN1 Antibody (PACO36818)
Antibody SKU:
PACO36818
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:1000-1:5000
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant Human Pyrin and HIN domain-containing protein 1 protein (1-245AA)
Western blot. All lanes: PYHIN1 antibody at 6µg/ml + Mouse small intestine tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 56, 55, 52, 51, 27, 26 kDa. Observed band size: 56, 22, 84 kDa.
Background:
Major mediator of the tumor suppressor activity of IFN in breast cancer cells. Promotes ubiquitination and subsequent degradation of MDM2, which leads to p53/TP53 stabilization. Promotes ubiquitination and subsequent degradation of HDAC1, which in turn enhances maspin expression, and impairs invasive activity of cancer cells.
Synonyms:
Pyrin and HIN domain-containing protein 1 (Interferon-inducible protein X), PYHIN1, IFIX
UniProt Protein Function:
Major mediator of the tumor suppressor activity of IFN in breast cancer cells. Promotes ubiquitination and subsequent degradation of MDM2, which leads to p53/TP53 stabilization. Promotes ubiquitination and subsequent degradation of HDAC1, which in turn enhances maspin expression, and impairs invasive activity of cancer cells.
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene belongs to the HIN-200 family of interferon-inducible proteins that share a 200-amino acid signature motif at their C-termini. HIN200 proteins are primarily nuclear and are involved in transcriptional regulation of genes important for cell cycle control, differentiation, and apoptosis. Downregulation of this gene is associated with breast cancer. This protein acts as a tumor suppressor by promoting ubiquitination and subsequent degradation of MDM2, which leads to stabilization of p53/TP53. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
