The PYGO2 Polyclonal Antibody (PACO58572) is a powerful tool for researchers studying the PYGO2 protein, which plays a key role in the Wnt signaling pathway. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blotting applications. By targeting the PYGO2 protein, this antibody allows for the precise detection and analysis of PYGO2 in a variety of cells, making it an essential tool for studies in cancer research and developmental biology.PYGO2 is a crucial component of the Wnt signaling pathway, which is involved in cell proliferation, migration, and differentiation.
Dysregulation of this pathway has been implicated in various diseases, including cancer and developmental disorders. By understanding the function of PYGO2, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the PACO58572 PYGO2 Polyclonal Antibody is a reliable reagent for researchers seeking to investigate the role of PYGO2 in biological processes and disease pathogenesis. Its high specificity and sensitivity make it a valuable asset in the quest for new treatments and interventions in the field of molecular biology.
IHC image of PACO58572 diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with PACO58572 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO58572 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Involved in signal transduction through the Wnt pathway.
