The PYGL Polyclonal Antibody (CAB6710) is a key tool for researchers studying PYGL, an enzyme involved in the regulation of glycogen metabolism. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blotting applications. By binding to the PYGL protein, this antibody allows for precise detection and analysis in various cell types, making it essential for investigations in metabolic disorders and related research fields.
PYGL, also known as glycogen phosphorylase L, plays a crucial role in breaking down glycogen to release glucose for energy production. Dysregulation of PYGL has been linked to metabolic diseases such as diabetes and glycogen storage disorders. Understanding the function and regulation of PYGL is essential for developing targeted therapies for these conditions, making the PYGL Polyclonal Antibody a valuable tool for researchers in the field of metabolism and metabolic disorders.
Product Name:
PYGL Rabbit Polyclonal Antibody
SKU:
CAB6710
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 690-847 of human PYGL (NP_002854.3).
cytoplasm, cytosol, extracellular exosome, extracellular region
Calculated MW:
97kDa
Observed MW:
97kDa
This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5836
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PYGL Rabbit pAb (CAB6710) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
