PYGL Rabbit Polyclonal Antibody (CAB6710)
- SKU:
- CAB6710
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
Product Name: | PYGL Rabbit Polyclonal Antibody |
SKU: | CAB6710 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 690-847 of human PYGL (NP_002854.3). |
Sequence: | IGTM DGAN VEMA EEAG EENL FIFG MRID DVAA LDKK GYEA KEYY EALP ELKL VIDQ IDNG FFSP KQPD LFKD IINM LFYH DRFK VFAD YEAY VKCQ DKVS QLYM NPKA WNTM VLKN IAAS GKFS SDRT IKEY AQNI WNVE PSDL KISL SNES NKVN GN |
Tested Applications: | WB IHC-P IF/ICC IP ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | GSD6; PYGL |
Positive Sample: | HeLa,HepG2,A-431,Mouse liver,Mouse kidney,Rat liver |
Conjugate: | Unconjugated |
Cellular Localization: | cytoplasm, cytosol, extracellular exosome, extracellular region |
Calculated MW: | 97kDa |
Observed MW: | 97kDa |
This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method: | Affinity purification |
Gene ID: | 5836 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |