The PYGB Polyclonal Antibody (CAB6402) is a valuable tool for researchers studying the enzyme PYGB, which plays a crucial role in glycogen metabolism. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blotting applications. By specifically binding to PYGB, researchers can easily detect and analyze the enzyme in various cell types, making it an ideal choice for studies in metabolism, diabetes, and other metabolic disorders.PYGB is an essential enzyme involved in breaking down glycogen, the main storage form of glucose in the body.
Dysregulation of PYGB activity can lead to metabolic disorders such as glycogen storage diseases and diabetes. By understanding the function and regulation of PYGB, researchers can gain insights into these diseases and potentially discover new therapeutic targets for treatment.Overall, the PYGB Polyclonal Antibody is a valuable tool for researchers interested in studying the role of PYGB in metabolism and metabolic disorders. Its high reactivity and specificity make it a reliable choice for Western blotting experiments, enabling detailed analysis of PYGB in various biological samples.
Product Name:
PYGB Rabbit Polyclonal Antibody
SKU:
CAB6402
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2).
cytoplasm, extracellular exosome, extracellular region
Calculated MW:
97kDa
Observed MW:
105kDa
The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation.
Purification Method:
Affinity purification
Gene ID:
5834
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PYGB Rabbit pAb (CAB6402) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
