The PYGB Polyclonal Antibody (CAB13539) is a valuable tool for research on PYGB, an important enzyme involved in glycogen metabolism. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the PYGB protein, allowing for accurate detection and analysis in a variety of cell types.PYGB is a key player in the breakdown of glycogen for energy production in cells, making it a crucial component in metabolic processes.
Dysregulation of PYGB activity has been linked to metabolic disorders such as diabetes and obesity, making it a target of interest for research in these areas. By studying PYGB with this antibody, researchers can gain insights into its functions and potential therapeutic applications in metabolic diseases.
Product Name:
PYGB Rabbit Polyclonal Antibody
SKU:
CAB13539
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2).
cytoplasm, extracellular exosome, extracellular region
Calculated MW:
97kDa
Observed MW:
100kDa
The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation.
Purification Method:
Affinity purification
Gene ID:
5834
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PYGB antibody (CAB13539) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
