The PYCR1 Polyclonal Antibody (CAB13346) is a valuable tool for researchers studying PYCR1, an enzyme involved in proline biosynthesis and cell metabolism. This antibody, raised in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding to the PYCR1 protein, this antibody facilitates detection and analysis in a variety of cell types, making it an essential resource for research in cell biology, metabolism, and cancer studies.PYCR1 is a key player in the synthesis of proline, an amino acid with crucial roles in protein structure and function, collagen formation, and cellular signaling pathways.
Dysregulation of PYCR1 has been implicated in various diseases, including cancer, metabolic disorders, and neurodegenerative conditions. By studying PYCR1 and its enzymatic activity, researchers can gain insights into disease mechanisms and potentially identify novel therapeutic targets for intervention.Overall, the PYCR1 Polyclonal Antibody is a reliable tool for investigating the role of PYCR1 in cellular metabolism and disease pathogenesis, allowing for valuable discoveries in the fields of biochemistry, cancer biology, and therapeutic development.
Product Name:
PYCR1 Rabbit Polyclonal Antibody
SKU:
CAB13346
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-319 of human PYCR1 (NP_008838.2).
This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5831
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of HepG2, using PYCR1 Rabbit pAb (CAB13346) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
