The PYCARD Polyclonal Antibody (CAB1170) is essential for researchers studying the PYCARD protein, which plays a key role in the innate immune response and inflammation. As a vital component of the inflammasome complex, PYCARD is involved in the activation of caspase-1 and the production of pro-inflammatory cytokines.Produced in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the PYCARD protein, researchers can effectively detect and analyze PYCARD expression in various cell types, making it an ideal tool for studies in immunology, inflammation, and infectious diseases.
The PYCARD Polyclonal Antibody is a valuable resource for understanding the mechanisms underlying immune responses and inflammatory processes. By investigating the role of PYCARD in disease states such as cancer, autoimmune disorders, and infectious diseases, researchers can uncover new therapeutic targets and develop innovative treatments to improve patient outcomes.
Product Name:
ASC/TMS1 Rabbit Polyclonal Antibody
SKU:
CAB1170
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-195 of human ASC/TMS1 (NP_037390.2).
This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
29108
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse spleen, using ASC/TMS1 Rabbit pAb (CAB1170) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
