The PYCARD Polyclonal Antibody (CAB11433) is a valuable tool for researchers studying the PYCARD protein, also known as apoptotic speck protein containing a caspase recruitment domain. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.The PYCARD protein plays a crucial role in inflammatory responses and cell death pathways, making it a key player in various cellular processes. By binding to PYCARD, this antibody enables the detection and analysis of the protein in various cell types, making it a versatile tool for studies in immunology and cell biology.
Researchers interested in understanding the intricate mechanisms of inflammatory signaling, cell death pathways, and immune responses will find the PYCARD Polyclonal Antibody to be a valuable asset in their research. With its high reactivity and specificity, this antibody offers reliable and accurate results, making it an essential component in studies related to inflammation, apoptosis, and related diseases.
Product Name:
ASC/TMS1 Rabbit Polyclonal Antibody
SKU:
CAB11433
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ASC/TMS1 (NP_037390.2).
Sequence:
MGRA RDAI LDAL ENLT AEEL KKFK LKLL SVPL REGY GRIP RGAL LSMD ALDL TDKL VSFY LETY GAEL TANV LRDM GLQE MAGQ LQAA THQG SGAA PAGI
This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
29108
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse spleen, using ASC/TMS1 Rabbit pAb (CAB11433) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
