The PVRL4 Polyclonal Antibody (CAB16149) is a valuable tool for researchers studying PVRL4, a cell adhesion molecule known to be involved in various physiological and pathological processes. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the PVRL4 protein, this antibody allows for the detection and analysis of PVRL4 expression in different cell types, making it an ideal choice for studies in cell biology, developmental biology, and cancer research.
PVRL4, also known as nectin-4, is a member of the nectin family of adhesion molecules that play a crucial role in cell-cell adhesion and communication. Aberrant expression of PVRL4 has been linked to cancer progression and metastasis, making it an attractive target for investigating tumor development and potential therapeutic interventions. By elucidating the functions of PVRL4, researchers can gain insights into the mechanisms underlying cancer and other diseases, paving the way for the development of targeted treatments and diagnostic tools.
Product Name:
PVRL4 Rabbit Polyclonal Antibody
SKU:
CAB16149
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 32-146 of human PVRL4 (NP_112178.2).
Cell junction, Cell membrane, Secreted, Single-pass type I membrane protein, adherens junction
Calculated MW:
55kDa
Observed MW:
62kDa/75kDa
This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.
Purification Method:
Affinity purification
Gene ID:
81607
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PVRL4 Rabbit pAb (CAB16149) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
