The PUS1 Polyclonal Antibody (CAB8720) is a research tool developed for studies involving PUS1, a protein involved in RNA modification and tRNA synthesis. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PUS1 protein, this antibody enables the detection and analysis of PUS1 in various cell types, making it suitable for investigations in RNA biology and molecular biology research.PUS1, a pseudouridine synthase, plays a crucial role in modifying RNA molecules by converting uridine residues to pseudouridine, thereby influencing RNA structure and function.
Understanding the functions and mechanisms of PUS1 is essential for unraveling its impact on RNA metabolism and cellular processes. Research on PUS1 has implications for studying RNA modifications, tRNA processing, and potentially uncovering new therapeutic targets for RNA-related disorders.Overall, the PUS1 Polyclonal Antibody (CAB8720) serves as a valuable tool for researchers interested in exploring the role of PUS1 in RNA biology, tRNA modification, and related molecular pathways. Its specificity and reliability make it an ideal choice for investigations into the functions of PUS1 in various biological contexts.
Product Name:
PUS1 Rabbit Polyclonal Antibody
SKU:
CAB8720
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 308-427 of human PUS1 (NP_079491.2).
This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
80324
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from U-87MG cells, using PUS1 Rabbit pAb (CAB8720) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
