The PUMA Rabbit Monoclonal Antibody (CAB3752) is a highly specific antibody designed for research involving PUMA, a pro-apoptotic protein involved in the regulation of cell death. This antibody, generated from rabbit monoclonal antibodies, is particularly reactive with human samples and has been validated for use in immunohistochemistry and flow cytometry applications.PUMA, also known as p53 upregulated modulator of apoptosis, plays a crucial role in the induction of apoptosis in response to various cellular stresses. Its dysregulation has been implicated in numerous diseases, including cancer and neurodegenerative disorders.
Therefore, studying the expression and activity of PUMA is essential for understanding its role in these pathological conditions and potentially identifying therapeutic targets.With its high specificity and sensitivity, the PUMA Rabbit Monoclonal Antibody (CAB3752) is an invaluable tool for researchers investigating cell death pathways, cancer biology, and the molecular mechanisms underlying disease progression. Its ability to accurately detect PUMA protein levels in various cell types makes it an essential reagent for studies aiming to elucidate the role of PUMA in health and disease.
Product Name:
PUMA Rabbit Monoclonal Antibody
SKU:
CAB3752
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-193 of human PUMA (NP_055232.1)
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
27113
Clone Number:
ARC0247
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using PUMA Rabbit mAb (CAB3752) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
