The PUM1 Polyclonal Antibody (CAB13389) is a valuable tool for researchers studying the PUM1 protein, which plays a key role in post-transcriptional regulation of gene expression. This antibody, developed using rabbit immunization techniques, shows high reactivity with human samples and is validated for use in Western blot applications.PUM1 is a member of the PUF family of RNA-binding proteins, known for their role in mRNA decay and translational repression. By targeting the PUM1 protein, researchers can gain insights into its functions in RNA metabolism and its involvement in various biological processes. This antibody enables precise detection and analysis of PUM1 in different cell types, making it suitable for studies in molecular biology and developmental research.
The PUM1 Polyclonal Antibody is a valuable tool for investigating the regulatory mechanisms controlled by the PUM1 protein. Understanding the role of PUM1 in post-transcriptional gene regulation is essential for unraveling its contributions to cellular processes such as proliferation, differentiation, and apoptosis. Researchers can leverage this antibody to advance their understanding of RNA-mediated gene expression control and its implications in various physiological and pathological conditions.
Product Name:
Pumilio 1 Rabbit Polyclonal Antibody
SKU:
CAB13389
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human Pumilio 1 (NP_055491.1).
This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
9698
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Pumilio 1 antibody (CAB13389) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
