The PUF60 Polyclonal Antibody (CAB6709) is a valuable tool for researchers studying the PUF60 protein, which is known for its involvement in pre-mRNA splicing and RNA binding activity. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PUF60 protein, this antibody enables accurate detection and analysis in various cell types, making it well-suited for research in molecular biology and gene expression studies.PUF60, also referred to as poly-U binding splicing factor 60, plays a crucial role in regulating gene expression through its interactions with RNA molecules.
Understanding the function and mechanisms of PUF60 is essential for unraveling the complexities of RNA processing and splicing, which have implications for a wide range of biological processes. Research on PUF60 can provide insights into diseases such as cancer, neurological disorders, and developmental abnormalities, where dysregulation of RNA splicing contributes to pathogenesis.By utilizing the PUF60 Polyclonal Antibody (CAB6709), researchers can delve deeper into the functions and interactions of the PUF60 protein, advancing our understanding of its role in gene regulation and its potential as a therapeutic target in various disease contexts.
Product Name:
PUF60 Rabbit Polyclonal Antibody
SKU:
CAB6709
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2).
This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
22827
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PUF60 Rabbit pAb (CAB6709) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
