The PTRF Polyclonal Antibody (CAB18331) is a powerful tool for research involving PTRF, a protein essential for caveolae formation and maintenance. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to PTRF, this antibody enables the detection and analysis of the protein in various cell types, making it an invaluable asset for studies in cell biology, lipid metabolism, and signaling pathways.PTRF, also known as Polymerase I and Transcript Release Factor, plays a crucial role in the organization and function of caveolae, lipid-rich domains on the plasma membrane. These structures are involved in diverse cellular processes such as endocytosis, signal transduction, and lipid homeostasis.
Research into PTRF function is essential for understanding its role in cellular physiology and its implications for diseases like metabolic disorders, cardiovascular diseases, and cancer.By utilizing the PTRF Polyclonal Antibody, researchers can explore the intricate mechanisms underlying caveolae formation and function, leading to a deeper understanding of cellular processes and potential therapeutic targets. Its high specificity and sensitivity make it an ideal tool for investigating the complex interplay between PTRF, caveolae, and various cellular pathways.
Product Name:
PTRF Rabbit Polyclonal Antibody
SKU:
CAB18331
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 200-280 of human PTRF (NP_036364.2).
This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.
Purification Method:
Affinity purification
Gene ID:
284119
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PTRF Rabbit pAb (CAB18331) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
