The PTPRF Polyclonal Antibody (CAB5444) is a valuable tool for researchers studying PTPRF, a protein tyrosine phosphatase family member involved in cell signaling and regulation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the PTPRF protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in the fields of cell biology and signal transduction.
PTPRF is known to play a crucial role in various cellular processes, such as cell growth, differentiation, and migration, making it a promising target for research into diseases like cancer, diabetes, and neurodegenerative disorders. Understanding the function and regulation of PTPRF is essential for the development of targeted therapies that could potentially benefit patients suffering from these conditions.
Product Name:
PTPRF Rabbit Polyclonal Antibody
SKU:
CAB5444
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1016-1250 of human PTPRF (NP_002831.2).
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.
Purification Method:
Affinity purification
Gene ID:
5792
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from bewo cells, using PTPRF Rabbit pAb (CAB5444) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
