CD45 Rabbit Polyclonal Antibody (CAB0372)
- SKU:
- CAB0372
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Product Name: | CD45 Rabbit Polyclonal Antibody |
| SKU: | CAB0372 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 611-730 of human CD45 (NP_002829.3). |
| Sequence: | NLDE QQEL VERD DEKQ LMNV EPIH ADIL LETY KRKI ADEG RLFL AEFQ SIPR VFSK FPIK EARK PFNQ NKNR YVDI LPYD YNRV ELSE INGD AGSN YINA SYID GFKE PRKY IAAQ GPRD |
| Tested Applications: | WB IF/ICC IP ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Synonyms: | LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; IMD105 |
| Positive Sample: | Jurkat |
| Conjugate: | Unconjugated |
| Cellular Localization: | Membrane, Membrane raft, Single-pass type I membrane protein. |
| Calculated MW: | 147kDa |
| Observed MW: | 200-250kDa |
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
| Purification Method: | Affinity purification |
| Gene ID: | 5788 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Jurkat cells, using CD45 Rabbit pAb (CAB0372) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s. |
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