The PTPRC Polyclonal Antibody (CAB0372) is a vital tool for researchers studying PTPRC, a protein also known as CD45 that plays a crucial role in immune cell signaling and activation. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications. By binding to the PTPRC protein, this antibody enables accurate detection and analysis in a variety of cell types, particularly in studies focusing on immunology and cancer research.
PTPRC, or CD45, is a key regulator of immune responses and is involved in lymphocyte development and activation. Dysregulation of PTPRC has been implicated in various autoimmune diseases, cancers, and immune system disorders, making it a valuable target for therapeutic interventions. Understanding the function and expression of PTPRC is essential for unraveling the complexities of immune responses and developing targeted treatments for a range of diseases.
Product Name:
CD45 Rabbit Polyclonal Antibody
SKU:
CAB0372
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 611-730 of human CD45 (NP_002829.3).
Sequence:
NLDE QQEL VERD DEKQ LMNV EPIH ADIL LETY KRKI ADEG RLFL AEFQ SIPR VFSK FPIK EARK PFNQ NKNR YVDI LPYD YNRV ELSE INGD AGSN YINA SYID GFKE PRKY IAAQ GPRD
Tested Applications:
WBIF/ICCIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Membrane, Membrane raft, Single-pass type I membrane protein.
Calculated MW:
147kDa
Observed MW:
200-250kDa
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Purification Method:
Affinity purification
Gene ID:
5788
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using CD45 Rabbit pAb (CAB0372) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
