The PTGER1 Polyclonal Antibody (CAB2913) is specifically designed for research involving the PTGER1 protein, which is a G protein-coupled receptor known to be involved in various physiological processes, including inflammation and immune response modulation. The antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. Its binding capability enables the detection and analysis of PTGER1 in different cell types, making it an ideal tool for studies in immunology and inflammatory diseases.
PTGER1, also known as EP1, plays a critical role in mediating the inflammatory response by modulating the production of pro-inflammatory mediators. Its involvement in immune regulation makes it a potential target for research into diseases such as arthritis, asthma, and autoimmune disorders. Understanding the function of PTGER1 is crucial for the development of therapies aimed at modulating inflammation and immune responses in these pathological conditions.
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PGE receptor EP1 (PTGER1) (P34995).
Sequence:
VYIL LRQA VLRQ LLRL LPPR AGAK GGPA GLGL TPSA WEAS SLRS SRHS GLSH F
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
EP1; PGE receptor EP1 (PTGER1)
Positive Sample:
Mouse kidney
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Multi-pass membrane protein.
Calculated MW:
42kDa
Observed MW:
42kDa
The protein encoded by this gene is a member of the G protein-coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). Through a phosphatidylinositol-calcium second messenger system, G-Q proteins mediate this receptor's activity. Knockout studies in mice suggested a role of this receptor in mediating algesia and in regulation of blood pressure. Studies in mice also suggested that this gene may mediate adrenocorticotropic hormone response to bacterial endotoxin.
Purification Method:
Affinity purification
Gene ID:
5731
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse kidney, using PGE receptor EP1 (PTGER1) Rabbit pAb (CAB2913) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).
