PTF1A Antibody (PACO51902)
- SKU:
- PACO51902
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
PTF1A Antibody (PACO51902)
The PTF1A Polyclonal Antibody (PAC051902) is a valuable tool for researchers studying the PTF1A protein, a transcription factor known for its role in pancreatic development and function. Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the PTF1A protein, this antibody enables accurate detection and analysis in various cell types, making it an essential tool for investigations in developmental biology and pancreatic cancer research.PTF1A is a key player in pancreatic development, controlling the expression of genes involved in pancreatic morphogenesis and differentiation.
Dysregulation of PTF1A has been linked to pancreatic diseases such as pancreatic agenesis and pancreatic cancer, highlighting the importance of studying this transcription factor in both normal and pathological conditions. Research on PTF1A provides valuable insights into the molecular mechanisms underlying pancreatic development and disease, paving the way for potential therapeutic interventions targeting these pathways.
| Antibody Name: | PTF1A Antibody (PACO51902) |
| Antibody SKU: | PACO51902 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant Human Pancreas transcription factor 1 subunit α protein (224-325AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Mouse brain tissue, Rat liver tissue. All lanes: PTF1A antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 35 kDa. Observed band size: 35 kDa.. |
 | Immunofluorescent analysis of Hela cells using PACO51902 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human pancreatic tissue using PACO51902 at dilution of 1:100. |
| Background: | Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Directly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates. |
| Synonyms: | Pancreas transcription factor 1 subunit α (Class A basic helix-loop-helix protein 29) (bHLHa29) (Pancreas-specific transcription factor 1a) (bHLH transcription factor p48) (p48 DNA-binding subunit of transcription factor PTF1) (PTF1-p48), PTF1A, BHLHA29 PTF1P48 |
| UniProt Protein Function: | PTF1A: Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH). |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 10p12.2 Cellular Component: cytoplasm; nucleus; transcription factor complex Molecular Function:DNA binding Biological Process: cerebellum development; exocrine pancreas development; pancreas development; regulation of transcription, DNA-dependent; tissue development Disease: Pancreatic Agenesis 2; Pancreatic And Cerebellar Agenesis |
| NCBI Summary: | This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q7RTS3 |
| NCBI GenInfo Identifier: | 74749931 |
| NCBI Gene ID: | 256297 |
| NCBI Accession: | Q7RTS3.1 |
| UniProt Secondary Accession: | Q7RTS3,Q9HC25, |
| UniProt Related Accession: | Q7RTS3 |
| Molecular Weight: | 34,970 Da |
| NCBI Full Name: | Pancreas transcription factor 1 subunit alpha |
| NCBI Synonym Full Names: | pancreas specific transcription factor, 1a |
| NCBI Official Symbol: | PTF1AÂ Â |
| NCBI Official Synonym Symbols: | PACA; PAGEN2; bHLHa29; PTF1-p48Â Â |
| NCBI Protein Information: | pancreas transcription factor 1 subunit alpha |
| UniProt Protein Name: | Pancreas transcription factor 1 subunit alpha |
| UniProt Synonym Protein Names: | Class A basic helix-loop-helix protein 29; bHLHa29; Pancreas-specific transcription factor 1a; bHLH transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1; PTF1-p48 |
| Protein Family: | Pancreas transcription factor |
| UniProt Gene Name: | PTF1AÂ Â |
| UniProt Entry Name: | PTF1A_HUMAN |
| ELISA Kits |
| Human Pancreas Specific Transcription Factor 1 alpha / PTF1A ELISA Kit |
| Human PTF1 alpha (Pancreas Specific Transcription Factor 1 alpha) CLIA Kit (HUES01173) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
Related Products
Human Pancreas Specific Transcription Factor 1a (PTF1a) ELISA Kit
