The PTEN Polyclonal Antibody (CAB2113) is a valuable tool for researchers studying the PTEN protein, a tumor suppressor that plays a key role in regulating cell growth and division. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.PTEN, also known as phosphatase and tensin homolog, is essential for maintaining genomic stability and preventing uncontrolled cell proliferation. Mutations in the PTEN gene are commonly found in various cancers, making it a significant target for cancer research.
By detecting and analyzing PTEN levels in different cell types, this antibody facilitates investigations into the role of PTEN in cancer development and progression.Understanding the functions and regulatory mechanisms of PTEN is crucial for developing targeted therapies for cancer and other diseases associated with PTEN dysregulation. The PTEN Polyclonal Antibody (CAB2113) provides researchers with a reliable tool to explore the complex biology of PTEN and its implications for disease pathology.
Product Name:
PTEN Rabbit Polyclonal Antibody
SKU:
CAB2113
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 190-304 of human PTEN (NP_000305.3).
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5728
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded rat brain using PTEN Rabbit pAb (CAB2113) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
