The PTEN Polyclonal Antibody (CAB11193) is a research tool designed for studies involving PTEN, a tumor suppressor gene that plays a crucial role in regulating cell growth and division. This antibody, generated in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the PTEN protein, allowing for accurate detection and analysis in a variety of cell types, making it well-suited for investigations in cancer research and molecular biology.PTEN, also known as phosphatase and tensin homolog, is a key player in the PI3K/AKT signaling pathway, which is involved in cell survival, growth, and proliferation. Dysregulation of this pathway, often due to mutations or loss of PTEN function, has been linked to various cancers and other diseases.
As such, studying PTEN expression and activity is critical for understanding the mechanisms underlying these conditions and for developing targeted therapies.The PTEN Polyclonal Antibody serves as a valuable tool for researchers looking to explore the role of PTEN in cancer progression, drug resistance, and tumor development. By enabling accurate detection and quantitation of PTEN protein levels, this antibody facilitates detailed investigations into the molecular pathways affected by PTEN dysregulation, providing valuable insights for the development of novel treatment strategies.
Product Name:
[KO Validated] PTEN Rabbit Polyclonal Antibody
SKU:
CAB11193
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PTEN (NP_000305.3).
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5728
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using PTEN Rabbit pAb (CAB11193) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
