PTEN Rabbit Polyclonal Antibody (CAB11189)
- SKU:
- CAB11189
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
Product Name: | PTEN Rabbit Polyclonal Antibody |
SKU: | CAB11189 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 224-403 of human PTEN (NP_000305.3). |
Sequence: | IYSS NSGP TRRE DKFM YFEF PQPL PVCG DIKV EFFH KQNK MLKK DKMF HFWV NTFF IPGP EETS EKVE NGSL CDQE IDSI CSIE RADN DKEY LVLT LTKN DLDK ANKD KANR YFSP NFKV KLYF TKTV EEPS NPEA SSST SVTP DVSD NEPD HYRY SDTT DSDP ENEP FDED QHTQ ITKV |
Tested Applications: | WB IHC-P ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:100 |
Synonyms: | BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta; PTEN |
Positive Sample: | 293T,MCF7,NIH/3T3,SH-SY5Y |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm, Nucleus, PML body, Secreted. |
Calculated MW: | 47kDa |
Observed MW: | 47kDa |
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Purification Method: | Affinity purification |
Gene ID: | 5728 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |