The PTEN Polyclonal Antibody (CAB11189) is a valuable tool for researchers studying PTEN, a tumor suppressor gene that plays a key role in regulating cell growth and division. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the PTEN protein, this antibody allows for the detection and analysis of PTEN expression in various cell types, making it an essential component of studies in cancer research and beyond.PTEN, also known as phosphatase and tensin homolog, is a crucial player in the PI3K/AKT signaling pathway, which is frequently dysregulated in cancer and other diseases.
Understanding the role of PTEN in cell signaling and tumorigenesis is essential for developing targeted therapies and biomarkers for various types of cancer. The PTEN Polyclonal Antibody provides researchers with a reliable tool to investigate the function and expression of PTEN in both normal and pathological conditions, ultimately leading to advancements in the field of molecular biology and personalized medicine.
Product Name:
PTEN Rabbit Polyclonal Antibody
SKU:
CAB11189
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 224-403 of human PTEN (NP_000305.3).
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5728
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PTEN Rabbit pAb (CAB11189) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
