The PTEN Monoclonal Antibody is a powerful tool for researchers studying PTEN, a tumor suppressor protein known for its role in controlling cell growth and division. This monoclonal antibody, produced in mice, is highly specific to PTEN and has been validated for use in applications such as Western blot and immunohistochemistry.PTEN is a crucial player in the regulation of cell proliferation and survival, and its dysregulation has been linked to various cancers and other diseases.
By targeting PTEN with this monoclonal antibody, researchers can gain valuable insights into the protein's function and its involvement in different cellular processes.Whether studying cancer biology, signal transduction pathways, or therapeutic targeting, the PTEN Monoclonal Antibody offers reliable and accurate detection of PTEN in various sample types. With its high specificity and sensitivity, this antibody is a valuable asset for advancing research in oncology and beyond.
Product Name:
PTEN Monoclonal Antibody
SKU:
CAB21892
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 304-403 of human PTEN (P60484).
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5728
Clone Number:
ARC0364
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PTEN Rabbit mAb antibody (CAB21892) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
