The PTCH2 Polyclonal Antibody (PACO56262) is an essential tool for researchers studying the PTCH2 protein, a key player in the Hedgehog signaling pathway. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. By binding to PTCH2, this antibody enables precise detection and analysis of the protein in a variety of cell types, facilitating research in developmental biology and cancer biology.
PTCH2 serves as a receptor for Hedgehog proteins and plays a crucial role in controlling cell growth and differentiation. Dysregulation of the Hedgehog pathway, often involving mutations in PTCH2, is associated with various types of cancers and developmental disorders. By investigating the function and regulation of PTCH2 using this antibody, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for treatment.
IHC image of PACO56262 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of 293 cells with PACO56262 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
May have a role in epidermal development. May act as a receptor for Sonic hedgehog (SHH).
Synonyms:
Protein patched homolog 2 (PTC2), PTCH2
UniProt Protein Function:
PTCH2: May have a role in epidermal development. May act as a receptor for Sonic hedgehog (SHH). Belongs to the patched family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integral; Membrane protein, multi-passChromosomal Location of Human Ortholog: 1p34.1Cellular Component: integral to membraneMolecular Function: hedgehog receptor activity; smoothened bindingBiological Process: skin development; epidermis development; negative regulation of smoothened signaling pathway; signal transduction; hair cycleDisease: Basal Cell Nevus Syndrome; Basal Cell Carcinoma, Susceptibility To, 1; Medulloblastoma
UniProt Protein Details:
NCBI Summary:
This gene encodes a transmembrane receptor of the patched gene family. The encoded protein may function as a tumor suppressor in the hedgehog signaling pathway. Alterations in this gene have been associated with nevoid basal cell carcinoma syndrome, basal cell carcinoma, medulloblastoma, and susceptibility to congenital macrostomia. Alternatively spliced transcript variants have been described.[provided by RefSeq, Oct 2009]
