The PTCH1 Polyclonal Antibody (CAB14772) is a powerful tool for investigating the PTCH1 protein, a key player in the Hedgehog signaling pathway that is essential for embryonic development and tissue homeostasis. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for Western blot applications.PTCH1 is a transmembrane receptor protein that serves as a negative regulator of the Hedgehog pathway, controlling the activity of downstream signaling molecules. Dysregulation of PTCH1 has been implicated in various cancers, developmental disorders, and degenerative diseases, highlighting its importance in cellular growth and differentiation processes.
By targeting PTCH1 with this antibody, researchers can gain valuable insights into its function and potential therapeutic applications in these pathological conditions.Overall, the PTCH1 Polyclonal Antibody (CAB14772) is a valuable research tool for studying the molecular mechanisms underlying Hedgehog signaling and its implications in disease pathology. Its high reactivity and reliability make it a trusted companion for scientists working in the fields of developmental biology, oncology, and regenerative medicine.
Product Name:
PTCH1 Rabbit Polyclonal Antibody
SKU:
CAB14772
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 770-970 of human PTCH1 (NP_000255.2).
This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly.
Purification Method:
Affinity purification
Gene ID:
5727
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat skeletal muscle, using PTCH1 Rabbit pAb (CAB14772) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
