The PTBP1 Polyclonal Antibody (CAB1831) is a valuable tool for researchers studying PTBP1, a key protein involved in regulating alternative splicing of pre-mRNA. This antibody, raised in rabbits, has been specifically validated for its reactivity with human samples and is suitable for use in Western blot applications. By binding to PTBP1, this antibody enables researchers to detect and analyze the protein in a variety of cell types, making it an essential tool for studies in RNA biology and gene expression regulation.PTBP1, also known as polypyrimidine tract-binding protein 1, plays a crucial role in controlling gene expression by determining which exons are included or excluded during mRNA processing.
Dysregulation of PTBP1 has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic conditions. Understanding the function of PTBP1 is essential for developing targeted therapies that may correct aberrant splicing patterns and restore normal gene expression.Overall, the PTBP1 Polyclonal Antibody (CAB1831) offers researchers a reliable and accurate tool for investigating the role of PTBP1 in cellular processes and disease development, paving the way for advancements in therapeutic strategies and personalized medicine.
Product Name:
PTBP1 Rabbit Polyclonal Antibody
SKU:
CAB1831
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PTBP1 (NP_114368.1).
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
5725
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KO Validated] PTBP1 Rabbit pAb (CAB1831) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
