The PSPC1 Polyclonal Antibody (CAB9209) is a valuable tool for researchers studying the PSPC1 protein, a key player in RNA processing and gene expression regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PSPC1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an excellent choice for studies in molecular biology and gene regulation research.
PSPC1 is involved in a wide range of cellular processes, including mRNA splicing, transport, and localization. Its role in gene expression control makes it a crucial target for research in fields such as developmental biology, neurobiology, and cancer biology. By understanding the function and regulation of PSPC1, researchers can gain insights into various diseases and potentially identify novel therapeutic targets for intervention.
Product Name:
PSPC1 Rabbit Polyclonal Antibody
SKU:
CAB9209
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human PSPC1 (NP_001035879.1).
Sequence:
MMLR GNLK QVRI EKNP ARLR ALES AVGE SEPA AAAA MALA LAGE PAPP APAP PEDH PDEE MGFT IDIK SF
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
This gene encodes a nucleolar protein that localizes to punctate subnuclear structures that occur close to splicing speckles, known as paraspeckles. These paraspeckles are composed of RNA-protein structures that include a non-coding RNA, NEAT1/Men epsilon/beta, and the Drosophila Behavior Human Splicing family of proteins, which include the product of this gene and the P54NRB/NONO and PSF/SFPQ proteins. Paraspeckles may function in the control of gene expression via an RNA nuclear retention mechanism. The protein encoded by this gene is found in paraspeckles in transcriptionally active cells, but it localizes to unique cap structures at the nucleolar periphery when RNA polymerase II transcription is inhibited, or during telophase. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene, which is also located on chromosome 13, has been identified.
Purification Method:
Affinity purification
Gene ID:
55269
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PSPC1 Rabbit pAb (CAB9209) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
