The PSME3 Polyclonal Antibody is a valuable tool for research involving the PSME3 protein, a crucial component of the immunoproteasome complex. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PSME3 protein, this antibody allows for the detection and analysis of PSME3 in various cell types, making it an essential asset for studies in immunology and cancer research.PSME3, also known as proteasome activator complex subunit 3, plays a vital role in regulating protein degradation within cells. Dysregulation of the proteasome system, including PSME3, has been associated with various diseases, making it a promising target for therapeutic intervention.
By understanding the function and regulation of PSME3, researchers can gain insights into its role in disease pathology and potentially develop targeted therapies to modulate its activity.Overall, the PSME3 Polyclonal Antibody offers researchers a reliable and specific tool for studying the expression and function of PSME3 in a variety of research settings. Its versatility and usability in Western blot applications make it a valuable resource for advancing our understanding of proteasome function and its implications in disease biology.
Product Name:
PSME3 Polyclonal Antibody
SKU:
CAB21319
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human PSME3 (NP_005780.2).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
10197
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PSME3 Rabbit pAb (CAB21319) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
