The PSME1 Rabbit Monoclonal Antibody (CAB5070) is a powerful tool for researchers studying the PSME1 protein, also known as proteasome activator complex subunit 1. This antibody, developed in rabbits, is highly specific and reactive with human samples, making it an ideal choice for Western blot applications.PSME1 is a key component of the proteasome activator complex, which is responsible for regulating protein degradation and turnover within cells. Dysregulation of this process has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. By targeting PSME1, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.
With its high specificity and sensitivity, the PSME1 Rabbit Monoclonal Antibody (CAB5070) enables precise detection and analysis of PSME1 expression in various cell types and tissues. Its reliability and reproducibility make it an essential tool for studies in cell biology, molecular biology, and drug discovery. By utilizing this antibody, researchers can advance their understanding of proteasome function and its role in health and disease.
Product Name:
PSME1 Rabbit Monoclonal Antibody
SKU:
CAB5070
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human PSME1(NP_006254.1).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5720
Clone Number:
ARC1254
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PSME1 Rabbit mAb (CAB5070) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
