The PSMD8 Polyclonal Antibody (CAB6955) is an essential tool for researchers studying the PSMD8 protein, a key component of the 26S proteasome complex responsible for protein degradation. This antibody, produced in rabbits, boasts high reactivity with human samples and is validated for Western blot applications. By binding specifically to the PSMD8 protein, this antibody facilitates the detection and analysis of PSMD8 in a variety of cell types, making it a valuable asset for investigations in proteasome function, protein turnover, and cellular regulation.PSMD8, also known as Rpn12, plays a crucial role in maintaining cellular homeostasis by ensuring the timely and efficient degradation of proteins.
Dysregulation of PSMD8 activity has been implicated in various diseases, including neurodegenerative disorders, cancer, and inflammatory conditions. By elucidating the function of PSMD8, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention.Overall, the PSMD8 Polyclonal Antibody (CAB6955) is a reliable tool for researchers seeking to delve into the intricate workings of the proteasome complex and its role in health and disease. Its specificity and sensitivity make it an indispensable asset for studies aimed at unraveling the molecular mechanisms governing protein degradation and cellular physiology.
Product Name:
PSMD8 Rabbit Polyclonal Antibody
SKU:
CAB6955
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 101-350 of human PSMD8 (NP_002803.2).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1.
Purification Method:
Affinity purification
Gene ID:
5714
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PSMD8 antibody (CAB6955) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
