The PSMD4 Polyclonal Antibody (CAB1061) is a valuable tool for researchers studying the PSMD4 protein, a subunit of the 26S proteasome complex involved in protein degradation. This antibody, produced in rabbits, exhibits high specificity for human samples and is optimized for use in Western blot applications. By targeting the PSMD4 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in molecular biology and cancer research.PSMD4 is a key player in the ubiquitin-proteasome system, a crucial pathway for maintaining cellular protein homeostasis.
Dysregulation of this system has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. Understanding the function of PSMD4 is essential for uncovering the mechanisms underlying these pathologies and developing targeted therapies. The PSMD4 Polyclonal Antibody provides researchers with a powerful tool to explore the role of PSMD4 in health and disease.
Product Name:
PSMD4 Rabbit Polyclonal Antibody
SKU:
CAB1061
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human PSMD4 (NP_002801.1).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5; PSMD4
Positive Sample:
HUVEC,MCF7
Conjugate:
Unconjugated
Cellular Localization:
cytosol, nucleoplasm, nucleus
Calculated MW:
41kDa
Observed MW:
50kDa
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21.
Purification Method:
Affinity purification
Gene ID:
5710
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HUVEC cells, using PSMD4 Rabbit pAb (CAB1061) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
