The PSMD2 Polyclonal Antibody (CAB1999) is a vital tool for research involving the PSMD2 protein, a key component of the 26S proteasome complex responsible for degrading unwanted proteins in cells. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.PSMD2 is essential for the proper functioning of the proteasome system, which plays a crucial role in maintaining cellular homeostasis by degrading damaged or unnecessary proteins. Dysregulation of the proteasome system has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
Therefore, understanding the function and activity of PSMD2 is integral to advancing research in these areas.The PSMD2 Polyclonal Antibody enables the specific detection and analysis of PSMD2 protein in a variety of cell types, making it a valuable tool for studies in cell biology, molecular biology, and drug discovery. By targeting PSMD2, researchers can gain insights into the mechanisms underlying protein degradation and potentially identify new therapeutic strategies for treating proteasome-related diseases.
Product Name:
PSMD2 Rabbit Polyclonal Antibody
SKU:
CAB1999
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PSMD2 (NP_002799.3).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene.
Purification Method:
Affinity purification
Gene ID:
5708
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PSMD2 antibody (CAB1999) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
