Anti-PSMD13 Antibody (CAB12485)
- SKU:
- CAB12485
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Anti-PSMD13 Antibody (CAB12485)
The PSMD13 Polyclonal Antibody (CAB12485) is a crucial tool for researchers studying the proteasome, a complex cellular machinery involved in protein degradation. This antibody, derived from rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the PSMD13 protein, this antibody facilitates the detection and analysis of this essential component of the proteasome in various cell types.PSMD13, also known as Rpn9, is a subunit of the 26S proteasome, responsible for recognizing and unfolding proteins targeted for degradation.
Understanding the role of PSMD13 in protein degradation pathways is essential for unraveling the mechanisms underlying various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. By elucidating the function of PSMD13, researchers can identify potential therapeutic targets for interventions aimed at modulating protein turnover and cellular homeostasis.
| Antibody Name: | Anti-PSMD13 Antibody |
| Antibody SKU: | CAB12485 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PSMD13 (NP_002808.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, SW620, Mouse liver, Mouse testis, Mouse skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PSMD13 (NP_002808.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKDV PGFL QQSQ NSGP GQPA VWHR LEEL YTKK LWHQ LTLQ VLDF VQDP CFAQ GDGL IKLY ENFI SEFE HRVN PLSL VEII LHVV RQMT DPNV ALTF LEKT REKV KSSD EAVI LCKT AIGA LKLN IGDL QVTK ETIE DVEE MLNN LPGV TSVH SRFY DLSS KYYQ TIGN HASY YKDA LRFL GCVD IKDL PVSE QQER AFTL GLAG LLGE GVFN FGEL LMHP VLES LRNT DRQW LIDT LYAF NSGN VERF QT |
| Gene ID: | 5719 |
| Uniprot: | Q9UNM6 |
| Cellular Location: | |
| Calculated MW: | 42kDa |
| Observed MW: | 43kDa |
| Synonyms: | PSMD13, HSPC027, Rpn9, S11, p40.5 |
| Background: | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. |
| UniProt Protein Function: | PSMD13: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the proteasome subunit S11 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protease; Proteasome complex Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: cytosol; extracellular region; membrane; nucleoplasm; nucleus; proteasome complex; proteasome regulatory particle Molecular Function:structural molecule activity Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; neutrophil degranulation; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; proteasome assembly; protein deubiquitination; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; transmembrane transport; tumor necrosis factor-mediated signaling pathway; ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway |
| NCBI Summary: | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UNM6 |
| NCBI GenInfo Identifier: | 317373273 |
| NCBI Gene ID: | 5719 |
| NCBI Accession: | Q9UNM6.2 |
| UniProt Secondary Accession: | Q9UNM6,O75831, Q53XU2, Q9UNV3, B3KT15, |
| UniProt Related Accession: | Q9UNM6 |
| Molecular Weight: | 43kDa |
| NCBI Full Name: | 26S proteasome non-ATPase regulatory subunit 13 |
| NCBI Synonym Full Names: | proteasome 26S subunit, non-ATPase 13 |
| NCBI Official Symbol: | PSMD13Â Â |
| NCBI Official Synonym Symbols: | S11; Rpn9; p40.5; HSPC027Â Â |
| NCBI Protein Information: | 26S proteasome non-ATPase regulatory subunit 13 |
| UniProt Protein Name: | 26S proteasome non-ATPase regulatory subunit 13 |
| UniProt Synonym Protein Names: | 26S proteasome regulatory subunit RPN9; 26S proteasome regulatory subunit S11; 26S proteasome regulatory subunit p40.5 |
| Protein Family: | 26S proteasome non-ATPase regulatory |
| UniProt Gene Name: | PSMD13Â Â |
 | Western blot analysis of extracts of various cell lines, using PSMD13 Antibody (CAB12485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s. |
 | Immunohistochemistry of paraffin-embedded rat brain using PSMD13 antibody (CAB12485) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophagus using PSMD13 antibody (CAB12485) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using PSMD13 antibody (CAB12485). Blue: DAPI for nuclear staining. |
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