The PSMD12 Polyclonal Antibody (CAB6708) is a specialized tool for researchers studying PSMD12, a key regulatory subunit of the proteasome complex involved in protein degradation. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it an invaluable resource in Western blot applications.PSMD12 is essential for the function of the proteasome, a cellular machinery responsible for maintaining protein homeostasis by degrading damaged or unwanted proteins. Dysregulation of PSMD12 can lead to various pathological conditions, including cancer, neurodegenerative diseases, and autoimmune disorders.
By targeting PSMD12 with this antibody, researchers can gain insights into the molecular mechanisms underlying these diseases and potentially identify new therapeutic targets.The PSMD12 Polyclonal Antibody is a reliable tool for the study of protein degradation pathways and the role of the proteasome complex in cellular physiology. Its versatility in detecting PSMD12 in various cell types makes it a valuable asset for investigations in cancer biology, neurobiology, and immunology. By using this antibody, researchers can advance our understanding of proteasome function and its implications for human health.
Product Name:
PSMD12 Rabbit Polyclonal Antibody
SKU:
CAB6708
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 197-456 of human PSMD12 (NP_002807.1).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5718
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PSMD12 antibody (CAB6708) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
