The PSMC5 Polyclonal Antibody (CAB13537) is a valuable tool for researchers studying PSMC5, a subunit of the 26S proteasome complex involved in protein degradation. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blotting applications. By targeting the PSMC5 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential tool for studies in molecular biology and cancer research.PSMC5 is a key player in the regulation of protein turnover and degradation, making it crucial for maintaining cellular homeostasis and preventing the accumulation of damaged or misfolded proteins.
Dysregulation of PSMC5 has been implicated in various diseases, including cancer and neurodegenerative disorders, highlighting its importance as a potential therapeutic target. By understanding the function of PSMC5, researchers can develop novel treatments that target the proteasome pathway and potentially combat these debilitating conditions.
Product Name:
PSMC5 Rabbit Polyclonal Antibody
SKU:
CAB13537
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 127-406 of human PSMC5 (NP_002796.4).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5705
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PSMC5 antibody (CAB13537) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
