The PSMC3 Polyclonal Antibody (CAB13345) is a valuable tool for researchers studying the PSMC3 protein, a subunit of the 26S proteasome complex involved in protein degradation and turnover. This antibody, produced through rabbit immunization, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the PSMC3 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal choice for investigations in molecular biology and cancer research.
PSMC3, also known as proteasomal 26S subunit ATPase 3, plays a crucial role in maintaining cellular homeostasis by facilitating the degradation of damaged or unnecessary proteins. Dysregulation of the proteasome complex, including PSMC3, has been associated with various diseases, such as cancer, neurodegenerative disorders, and autoimmune conditions. By studying the function and activity of PSMC3, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention.
Product Name:
PSMC3 Rabbit Polyclonal Antibody
SKU:
CAB13345
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PSMC3 (NP_002799.3).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
RPT5; TBP1; DCIDP; PSMC3
Positive Sample:
MCF7,Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, P-body.
Calculated MW:
49kDa
Observed MW:
50kDa
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.
Purification Method:
Affinity purification
Gene ID:
5702
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PSMC3 Rabbit pAb (CAB13345) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
