The PSMC2 Polyclonal Antibody (CAB1985) is a valuable tool for researchers studying the PSMC2 protein, a subunit of the 26S proteasome complex involved in regulating protein degradation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding specifically to the PSMC2 protein, this antibody enables the detection and analysis of PSMC2 expression in a variety of cell types, making it an essential reagent for studies in cell biology, protein degradation mechanisms, and disease pathways.PSMC2, a key player in the proteasome complex, is crucial for maintaining cellular homeostasis by degrading damaged or misfolded proteins.
Dysregulation of PSMC2 activity has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. By understanding the function and regulation of PSMC2, researchers can gain valuable insights into the molecular mechanisms underlying these diseases and potentially identify novel therapeutic targets for intervention. The PSMC2 Polyclonal Antibody is a reliable tool for investigating the role of PSMC2 in cellular processes and disease pathogenesis.
Product Name:
PSMC2 Rabbit Polyclonal Antibody
SKU:
CAB1985
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human PSMC2 (NP_002794.1).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
5701
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PSMC2 antibody (CAB1985) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
