The PSMC1 Polyclonal Antibody (CAB15712) is a crucial tool for researchers studying the PSMC1 protein, a key component of the 26S proteasome complex involved in protein degradation. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been verified for reliable use in Western blot applications.PSMC1, also known as the ATPase subunit of the 26S proteasome, plays a vital role in regulating protein turnover and maintaining cellular homeostasis. Dysregulation of PSMC1 has been implicated in various diseases, including neurodegenerative disorders, cancer, and inflammatory conditions.
By targeting PSMC1 with this antibody, researchers can investigate its role in disease pathogenesis and explore potential therapeutic strategies aimed at modulating proteasome activity.With its specificity and reliability, the PSMC1 Polyclonal Antibody (CAB15712) is an invaluable tool for advancing research in molecular biology, cell biology, and biomedical sciences. Its ability to detect and quantify PSMC1 expression in diverse cell types makes it a versatile asset for studies focused on understanding the complex mechanisms underlying protein degradation and cellular function.
Product Name:
PSMC1 Rabbit Polyclonal Antibody
SKU:
CAB15712
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 77-440 of human PSMC1 (NP_002793.2).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit and a 20S core alpha subunit interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. This subunit also interacts with the adenovirus E1A protein and this interaction alters the activity of the proteasome. Finally, this subunit interacts with ataxin-7, suggesting a role for the proteasome in the development of spinocerebellar ataxia type 7, a progressive neurodegenerative disorder.
Purification Method:
Affinity purification
Gene ID:
5700
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PSMC1 Rabbit pAb (CAB15712) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
