The PSMB8 Polyclonal Antibody from Assaygenie (catalog number: AG10108) is a valuable tool for researchers studying the PSMB8 protein, also known as Proteasome Subunit Beta Type-8. PSMB8 is a vital component of the immunoproteasome, playing a crucial role in antigen processing and presenting peptides to the immune system.This polyclonal antibody, generated in rabbits, exhibits high specificity and sensitivity towards human PSMB8 samples, making it ideal for various applications such as Western blot, immunohistochemistry, and ELISA. By targeting the PSMB8 protein, researchers can accurately detect and analyze its expression levels in different cell types and tissues, providing insights into its function in immune response regulation and disease development.
Given the significant role of PSMB8 in antigen presentation and immune surveillance, understanding its mechanisms of action is key to developing novel therapies for immune-related disorders, autoimmune diseases, and cancer. The PSMB8 Polyclonal Antibody is a reliable tool for investigating the biological functions and signaling pathways involving PSMB8, paving the way for innovative research in immunology and personalized medicine.
Product Name:
PSMB8 Polyclonal Antibody
SKU:
CAB21574
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 163-272 of human PSMB8 (NP_004150.1).
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
Purification Method:
Affinity purification
Gene ID:
5696
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PSMB8 Rabbit pAb (CAB21574) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
