The PSMB8 Polyclonal Antibody (CAB7340) is a valuable tool for researchers studying the PSMB8 protein, which is involved in the immune response and antigen processing. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the PSMB8 protein, this antibody enables the detection and analysis of PSMB8 in various cell types, making it ideal for investigations in immunology and cancer research.
PSMB8, also known as immunoproteasome subunit beta-5i, plays a crucial role in the immune system by processing antigens for presentation to T cells. Dysregulation of PSMB8 has been implicated in autoimmune diseases and cancer, making it a promising target for therapeutic interventions. Research into the function of PSMB8 is essential for developing treatments that modulate immune responses in these diseases and promote immune system balance.
Product Name:
PSMB8 Rabbit Polyclonal Antibody
SKU:
CAB7340
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 163-272 of human PSMB8 (NP_004150.1).
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
Purification Method:
Affinity purification
Gene ID:
5696
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PSMB8 Rabbit pAb (CAB7340) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
