The PSMB4 Polyclonal Antibody (CAB5697) is a valuable tool for research involving the PSMB4 protein, a key component of the 20S proteasome complex. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the PSMB4 protein, researchers can accurately detect and analyze its expression in various cell types, making it ideal for studies in cell biology, proteasome function, and protein degradation pathways.PSMB4 is essential for the proper function of the proteasome, a critical cellular machinery responsible for degrading unwanted or damaged proteins.
Dysregulation of the proteasome system has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. Therefore, understanding the role of PSMB4 in proteasome activity is vital for uncovering its implications in these pathologies and developing targeted therapies.Overall, the PSMB4 Polyclonal Antibody offers researchers a reliable tool for studying the biological functions of PSMB4 and its involvement in cellular processes, providing valuable insights into disease mechanisms and potential therapeutic interventions.
Product Name:
PSMB4 Rabbit Polyclonal Antibody
SKU:
CAB5697
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human PSMB4 (NP_002787.2).
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit.
Purification Method:
Affinity purification
Gene ID:
5692
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PSMB4 antibody (CAB5697) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
