The PSMB2 Polyclonal Antibody (CAB13630) is a vital tool for researchers studying the PSMB2 protein, a component of the 26S proteasome complex involved in protein degradation. This antibody, produced in rabbits, is specifically reactive with human samples and has been validated for use in Western blot applications. By binding to the PSMB2 protein, this antibody allows for the detection and analysis of PSMB2 expression in various cell types, making it ideal for studies in molecular biology and cell biology research.
PSMB2 plays a crucial role in maintaining cellular homeostasis by regulating the degradation of proteins involved in important cellular processes. Its involvement in protein turnover makes it a key target for research into diseases such as cancer, neurodegenerative disorders, and autoimmune conditions. Understanding the function of PSMB2 is essential for developing targeted therapies that aim to manipulate protein degradation pathways for therapeutic benefit.
Product Name:
PSMB2 Rabbit Polyclonal Antibody
SKU:
CAB13630
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human PSMB2 (NP_002785.1).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
HC7-I; PSMB2
Positive Sample:
22Rv1,U-937,K-562,U-87MG,HT-29,HeLa,A673,BT-474
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
23kDa
Observed MW:
23kDa
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5690
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PSMB2 Rabbit pAb (CAB13630) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
