The PSMB10 Polyclonal Antibody (CAB5452) is a valuable tool for researchers studying the PSMB10 protein, a key component of the proteasome complex involved in protein degradation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the PSMB10 protein, this antibody enables the detection and analysis of this important molecule in various cell types, making it an essential reagent for studies in cell biology, molecular biology, and drug discovery.PSMB10 is a subunit of the immunoproteasome, a specialized form of the proteasome that plays a crucial role in immune responses by degrading antigens for presentation to T cells.
Dysregulation of the immunoproteasome has been implicated in various diseases, including autoimmune disorders, infectious diseases, and cancer. Therefore, understanding the function of PSMB10 is essential for developing therapies that target the immune system and improve treatment outcomes in these conditions.By using the PSMB10 Polyclonal Antibody (CAB5452), researchers can gain valuable insights into the role of PSMB10 in health and disease, leading to potential new therapeutic strategies and advancements in the field of immunology and drug development.
Product Name:
PSMB10 Rabbit Polyclonal Antibody
SKU:
CAB5452
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human PSMB10 (NP_002792.1).
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.
Purification Method:
Affinity purification
Gene ID:
5699
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from THP-1 cells, using PSMB10 Rabbit pAb (CAB5452) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
