The PRSS16 Polyclonal Antibody (PAC05953) is a valuable tool for researchers studying PRSS16, a protein involved in various biological processes, including regulation of immune responses. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for Western blot applications. By specifically binding to the PRSS16 protein, this antibody allows for detection and analysis in different cell types, making it an excellent choice for studies in immunology and cancer research.
PRSS16, also known as serine protease 16, is known to be involved in processes such as immune regulation and proteolysis. Its role in immune response modulation suggests its potential as a target for research into diseases like cancer, autoimmune disorders, and chronic inflammatory conditions. Investigating the function of PRSS16 is essential for understanding its impact on these conditions and developing potential therapies that manipulate immune responses effectively.
Western Blot. Positive WB detected in: Rat liver tissue, Mouse liver tissue. All lanes: PRSS16 antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56 kDa. Observed band size: 56 kDa.
Immunofluorescence staining of Hela cells with PACO59453 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO59453 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Protease that may play a role in T-cell development.
PRSS16: Protease that may play a role in T-cell development. Belongs to the peptidase S28 family.Protein type: EC 3.4.-.-; ProteaseChromosomal Location of Human Ortholog: 6p21Cellular Component: endosome; lysosomeMolecular Function: dipeptidyl-peptidase activity; serine carboxypeptidase activityBiological Process: proteolysis
UniProt Protein Details:
NCBI Summary:
This gene encodes a serine protease expressed exclusively in the thymus. It is thought to play a role in the alternative antigen presenting pathway used by cortical thymic epithelial cells during the positive selection of T cells. The gene is found in the large histone gene cluster on chromosome 6, near the major histocompatibility complex (MHC) class I region. A second transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]
