PRSS16 Antibody (PACO59453)
- SKU:
- PACO59453
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | PRSS16 Antibody (PACO59453) |
| Antibody SKU: | PACO59453 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant Human Thymus-specific serine protease protein (352-489AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Rat liver tissue, Mouse liver tissue. All lanes: PRSS16 antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56 kDa. Observed band size: 56 kDa. |
 | Immunofluorescence staining of Hela cells with PACO59453 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO59453 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Protease that may play a role in T-cell development. |
| Synonyms: | Thymus-specific serine protease (EC 3.4) (Serine protease 16), PRSS16, TSSP |
| UniProt Protein Function: | PRSS16: Protease that may play a role in T-cell development. Belongs to the peptidase S28 family.Protein type: EC 3.4.-.-; ProteaseChromosomal Location of Human Ortholog: 6p21Cellular Component: endosome; lysosomeMolecular Function: dipeptidyl-peptidase activity; serine carboxypeptidase activityBiological Process: proteolysis |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a serine protease expressed exclusively in the thymus. It is thought to play a role in the alternative antigen presenting pathway used by cortical thymic epithelial cells during the positive selection of T cells. The gene is found in the large histone gene cluster on chromosome 6, near the major histocompatibility complex (MHC) class I region. A second transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NQE7 |
| NCBI GenInfo Identifier: | 13633990 |
| NCBI Gene ID: | 10279 |
| NCBI Accession: | Q9NQE7.2 |
| UniProt Secondary Accession: | Q9NQE7,O75416 |
| UniProt Related Accession: | Q9NQE7 |
| Molecular Weight: | 55,049 Da |
| NCBI Full Name: | Thymus-specific serine protease |
| NCBI Synonym Full Names: | protease, serine 16 |
| NCBI Official Symbol: | PRSS16Â Â |
| NCBI Official Synonym Symbols: | TSSPÂ Â |
| NCBI Protein Information: | thymus-specific serine protease |
| UniProt Protein Name: | Thymus-specific serine protease |
| UniProt Synonym Protein Names: | Serine protease 16 |
| Protein Family: | Thymus-specific serine protease |
| UniProt Gene Name: | PRSS16Â Â |
| UniProt Entry Name: | TSSP_HUMAN |