The PRPF8 Polyclonal Antibody (CAB6053) is a valuable tool for researchers studying the PRPF8 protein, an essential component of the spliceosome complex involved in pre-mRNA splicing. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the PRPF8 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for research in molecular biology and gene expression studies.PRPF8 plays a crucial role in splicing, a fundamental process in gene expression that is dysregulated in various diseases, including cancer and genetic disorders.
Understanding the function and regulation of PRPF8 is essential for unraveling the complexities of splicing and its implications for human health. The PRPF8 Polyclonal Antibody provides researchers with a reliable tool for investigating the role of PRPF8 in various cellular processes, offering insights that may lead to the development of novel therapeutic strategies targeting splicing-related diseases.
Product Name:
PRPF8 Rabbit Polyclonal Antibody
SKU:
CAB6053
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3).
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.
Purification Method:
Affinity purification
Gene ID:
10594
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRPF8 Rabbit pAb (CAB6053) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
