The PRPF8 Rabbit Monoclonal Antibody (CAB4575) is a valuable tool for researchers studying the PRPF8 protein, a crucial component of the spliceosome involved in pre-mRNA splicing. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to PRPF8, this antibody enables precise detection and analysis of the protein in various cell types, facilitating research in fields such as molecular biology, genetics, and RNA splicing mechanisms.
PRPF8, also known as Pre-mRNA-processing factor 8, plays a vital role in the splicing of precursor mRNA molecules, ensuring proper gene expression and protein synthesis. Dysregulation of PRPF8 has been linked to various genetic disorders and diseases, highlighting the importance of studying its function and regulation. Researchers in the fields of genomics, RNA biology, and developmental biology can benefit from using this antibody to further understand the role of PRPF8 in health and disease.
Product Name:
PRPF8 Rabbit Monoclonal Antibody
SKU:
CAB4575
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 2200-2335 of human PRPF8 (Q6P2Q9).
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.
Purification Method:
Affinity purification
Gene ID:
10594
Clone Number:
ARC1038
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRPF8 Rabbit mAb (CAB4575) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
