The PRPF4B Polyclonal Antibody (CAB14815) is a powerful tool for researchers studying PRPF4B, a key protein involved in pre-mRNA splicing. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for detection and analysis in Western blot applications. By targeting PRPF4B, researchers can explore the intricate mechanisms of RNA processing and gene expression regulation, providing valuable insights into various cellular processes and diseases related to splicing defects.
PRPF4B is a critical component of the spliceosome, the molecular machinery responsible for removing introns and joining exons during mRNA maturation. Dysregulation of pre-mRNA splicing has been linked to numerous disorders, including cancer, neurodegenerative diseases, and genetic disorders. By investigating the role of PRPF4B in splicing, researchers can uncover potential therapeutic targets and diagnostic markers for these conditions, ultimately advancing our understanding of RNA biology and disease mechanisms.
Product Name:
PRPF4B Rabbit Polyclonal Antibody
SKU:
CAB14815
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 550-760 of human PRPF4B (NP_003904.3).
Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs).
Purification Method:
Affinity purification
Gene ID:
8899
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRPF4B Rabbit pAb (CAB14815) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
