The PRPF40A Polyclonal Antibody (PACO61582) is a valuable tool for researchers studying PRPF40A, a key protein involved in pre-mRNA splicing. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PRPF40A protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for investigations in molecular biology and RNA processing research.PRPF40A is essential for the splicing of pre-mRNA, a crucial step in gene expression regulation.
Dysregulation of this process can lead to various diseases, including cancer and genetic disorders. By studying the function of PRPF40A, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets. The PRPF40A Polyclonal Antibody is a valuable tool for advancing our understanding of pre-mRNA splicing and its implications in human health.
Western Blot. Positive WB detected in: U251 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate. All lanes: PRPF40A antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 109, 106, 107, 11 kDa. Observed band size: 109 kDa.
IHC image of PACO61582 diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. May play a role in cytokinesis. May be involved in pre-mRNA splicing.
Synonyms:
Pre-mRNA-processing factor 40 homolog A, Fas ligand-associated factor 1, Formin-binding protein 11, Formin-binding protein 3, Huntingtin yeast partner A, Huntingtin-interacting protein 10, HIP-10, Huntingtin-interacting protein A, Renal carcinoma antigen NY-REN-6, PRPF40A, FBP11, FLAF1, FNBP3, HIP10, HYPA, HSPC225
UniProt Protein Function:
FNBP3: Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. May play a role in cytokinesis. May be involved in pre-mRNA splicing. Belongs to the PRPF40 family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: RNA processing; SpliceosomeChromosomal Location of Human Ortholog: 2q23.3Cellular Component: cytoplasm; membrane; nuclear matrix; nucleoplasm; snRNP U1Molecular Function: protein binding; RNA bindingBiological Process: cell migration; cytoskeleton organization and biogenesis; nuclear mRNA splicing, via spliceosome; regulation of cell shape; regulation of cytokinesis
